FIG. 5.

Processing likelihood compared to selected experimental T cell epitope maps. The selected studies excluded tetramer-guided mapping, but included a DFV2 study that did not discriminate CD4+ and CD8+ T cells. Experimentally observed epitopes were reassigned to the corresponding peptides of hypothetical sets (to equalize sampling across studies) and then compared to the processing likelihood. Experimental epitopes were discovered at a rate of 11% of tested peptides (31 epitopes of 276 peptides tested). Experimental epitopes were more than twofold enriched (p < 0.02) within the subset of peptides having high processing likelihood (above the 80th percentile), when based on the postfusion conformations (15 correct of 55 peptides), but not the prefusion conformations (8 correct of 55 peptides). The 80th percentile of peptides ranked by processing likelihood varies by protein (horizontal dashed line).