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. 2017 Sep 22;14:44. doi: 10.1186/s12977-017-0369-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Pre-binding neutralization activity. The neutralization activities of anti-CD4i mAbs against BaL (a) and JR-FL (b) before the attachment of virus and target cell were analyzed. The pseudo-virus infected the target cells after pre-incubation in the presence of the neutralizing mAbs. Anti-CD4bs mAb, b12 (10 μg/ml), anti-V3 mAb, KD-247 and anti-CD4i mAbs (100 μg/ml) were used. The neutralization activities of the IgGs (black) and scFvs (gray) were standardized by the amount of p24