Fig. 7.

Binding activities of anti-CD4i mAbs against gp120 mutants. The binding activities of anti-CD4i mAbs against gp120 from BaL WT and mutants were measured by gp120 capture ELISA. We used the monomeric gp120 of WT and eight mutants including V3 base (gp120 with truncated V3 loop tip), ΔV3 (gp120 with truncated V3 loop containing the base of V3), ΔV1V2 (gp120 with truncated V1 and V2 loops), ΔV1 (gp120 with truncated V1 loop), ΔV2 (gp120 with truncated V2 loop), ΔH1 (gp120 with truncated V1/V2 loops containing the stem of the loops), ΔH1–V3 base (gp120 with the deletions of ΔH1 and V3 base mutants) and ΔH1–ΔV3 (gp120 with the deletions of ΔH1 and ΔV3) as previously described [10]. The binding activities of anti-CD4i mAbs in the presence (orange) or absence (blue) of sCD4 and anti-CD4bs mAb, 49G2 (gray) are shown