Table 1.
Summary of anti-CD4i mAb binding to different gp120 mutants
| Protein | Binding | |||||||
|---|---|---|---|---|---|---|---|---|
| 916B2 | 17b | 25C4b | 12G10 | 4E9C | 917B11 | 5D6S | A32 | |
| wt | ||||||||
| Absence of sCD4 | +++ | +++ | +++ | ++ | +++ | +++ | ++ | ++ |
| Presence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | ++ |
| V3 base | ||||||||
| Absence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Presence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| ΔV3 | ||||||||
| Absence of sCD4 | − | − | − | − | − | − | + | + |
| Presence of sCD4 | +++ | + | − | − | + | ++ | +++ | ++ |
| ΔV12 | ||||||||
| Absence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Presence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| ΔV1 | ||||||||
| Absence of sCD4 | +++ | +++ | +++ | ++ | +++ | +++ | ++ | ++ |
| Presence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| ΔV2 | ||||||||
| Absence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Presence of sCD4 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| ΔH1 | ||||||||
| Absence of sCD4 | − | − | − | − | +++ | ++ | + | +++ |
| Presence of sCD4 | − | ++ | ++ | ++ | +++ | +++ | +++ | +++ |
| ΔH1–V3 base | ||||||||
| Absence of sCD4 | − | − | − | − | +++ | ++ | − | +++ |
| Presence of sCD4 | − | ++ | ++ | ++ | +++ | +++ | ++ | +++ |
| ΔH1–ΔV3 | ||||||||
| Absence of sCD4 | − | − | − | − | − | − | − | ++ |
| Presence of sCD4 | − | − | − | − | − | − | +++ | ++ |
Relative binding activities of anti-CD4i mAbs against each gp120 mutant with the binding of anti-CD4bs Ab
* −: < 0.05, +: > 0.05–0.2, ++: > 0.2–0.4, +++: > 0.4