. Author manuscript; available in PMC: 2017 Sep 23.

Published in final edited form as: Chem Rev. 2015 Nov 9;115(22):12546–12629. doi: 10.1021/acs.chemrev.5b00434

Table 3.

Cumulative results of three studies that examined substrate specificities of caspases (◆ indicates that the substrate is cleaved, × stands for not cleaved substrates and - not determined).

	Substrate cleavage Ac-X-X-X-Asp-reporter: AFC⁵⁵, pNA⁵⁶, AMAC⁵⁷
	Ref.	YVAD	WEHD	VDVAD	DEVD	LEVD	VEID	IETD	LEHD	AEVD
Caspase-1	55	-	-	-	-	-	-	-	-	-
	56	◆	-	×	◆	-	◆	◆	◆	-
	57	×	◆	×	×	◆	×	×	◆	×
Caspase-2	55	-	-	◆	×	-	×	×	◆	-
	56	×	-	◆	×	×	×	×	◆	-
	57	×	×	◆	×	×	×	×	×	×
Caspase-3	55	-	-	◆	◆	-	◆	◆	◆	-
	56	×	-	◆	◆	-	◆	×	×	-
	57	×	×	◆	◆	◆	◆	×	×	◆
Caspase-4	55	-	-	-	-	-	-	-	-	-
	56	-	-	-	-	-	-	-	-	-
	57	×	◆	×	×	◆	×	×	◆	×
Caspase-5	55	-	-	-	-	-	-	-	-	-
	56	-	-	-	-	-	-	-	-	-
	57	×	◆	×	×	◆	×	×	◆	×
Caspase-6	55	-	-	×	◆	-	◆	◆	◆	-
	56	×	-	×	◆	-	◆	◆	◆	-
	57	×	×	×	◆	◆	◆	◆	◆	◆
Caspase-7	55	-	-	◆	◆	-	×	×	×	-
	56	×	-	◆	◆	-	◆	×	×	-
	57	×	×	◆	◆	◆	×	×	×	◆
Caspase-8	55	-	-	×	◆	-	◆	◆	◆	-
	56	×	-	×	◆	-	◆	◆	◆	-
	57	×	×	×	◆	◆	◆	◆	◆	◆
Caspase-9	55	-	-	×	×	-	×	◆	◆	-
	56	×	-	×	◆	-	◆	◆	◆	-
	57	×	×	◆	◆	◆	◆	◆	◆	◆
Caspase-10	55	-	-	◆	◆	-	◆	◆	◆	-
	56	-	-	-	-	-	-	-	-	-
	57	×	×	◆	◆	◆	◆	◆	◆	×