Biotin |
Fast and specific, pH stable, high sensitivity, low price |
Time-consuming detection |
Aza-peptide ABP 20,395,397
|
Rhodamineand derivatives |
Readily excited |
Lack of cell permeability,low quantum yield |
Aza-peptide ABP, FLICA with CaspaTag |
Cyanine |
Stable in SPPS, stable in broad pH range, hydrophilic, DMSO stable, photo stable |
High price |
Peptide-bases ABP 13,19,428
|
Radiolabeling |
Small size, easy detection, high activity, high sensitivity, easy synthesis, chemical stability, use in biological samples in vivo
|
Short half-life, harmful, gel-based analysis |
Mainly isatin-based probes 334,335,338,398–401,403
|
Fluorescein derivatives |
High absorptivity, hydrophilic, inexpensive, stable in SPPS |
Light-sensitive, unstable in pH>7, lack cell permeability, more sensitive at lower pH |
Using in FLICA, FAM-probes 13,20
|