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. Author manuscript; available in PMC: 2017 Sep 23.
Published in final edited form as: Chem Rev. 2015 Nov 9;115(22):12546–12629. doi: 10.1021/acs.chemrev.5b00434

Table 44.

Collected tags which were used in caspases labeling. Adapted from 414

Tag Advantage Disadvantage Application + Ref.
Biotin Fast and specific, pH stable, high sensitivity, low price Time-consuming detection Aza-peptide ABP 20,395,397
Rhodamineand derivatives Readily excited Lack of cell permeability,low quantum yield Aza-peptide ABP, FLICA with CaspaTag
Cyanine Stable in SPPS, stable in broad pH range, hydrophilic, DMSO stable, photo stable High price Peptide-bases ABP 13,19,428
Radiolabeling Small size, easy detection, high activity, high sensitivity, easy synthesis, chemical stability, use in biological samples in vivo Short half-life, harmful, gel-based analysis Mainly isatin-based probes 334,335,338,398–401,403
Fluorescein derivatives High absorptivity, hydrophilic, inexpensive, stable in SPPS Light-sensitive, unstable in pH>7, lack cell permeability, more sensitive at lower pH Using in FLICA, FAM-probes 13,20