Table 2.
Primers used in quantitative real-time PCR analysis
| Target gene | Primer | Sequence (5’to3’) |
|---|---|---|
| Epidermal differentiation-specific protein | EDSP_F | GTGGAGCTGAAAATCGTCC |
| EDSP_R | CATCAATGTAAGTTTCTGTGCC | |
| Carboxypeptidase Z | CPZ_F | GATTAAGCCTGGTCGGGTAG |
| CPZ_R | TGGGTCGAACTGATCTAACG | |
| Cadherin-13 | CDH13_F | AAGCCTTTGGACTACGAGA |
| CDH13_R | ATGGACCTTCATTGACATCAC | |
| Macrophage-capping protein | CAPG_F | TGACTGAACACGGGAAGG |
| CAPG_R | CAATGCTGGAGATGACGC | |
| Coronin-1A | CORO1A_F | GCTTTGATGAACCATTGACG |
| CORO1A_R | TGTGACTCCTTGCTGCTGTAC | |
| Basement membrane-specific heparan sulfate proteoglycan core protein | HSPG2_F | CAATACTGTGAGCGATGTGC |
| HSPG2_R | ACTTGAACGATGAAAGGAGC | |
| Annexin A1 | ANXA1_F | TTACGAAACTATTCTGGTGGC |
| ANXA1_R | TTTTGGGATTACAGGCACA | |
| eukaryotic translation initiation factor 5A isoform II | EIF5A2_F | AAGATACGAGGCAGGAGAAG |
| EIF5A2_R | AAGTGGGCGATTATTTGGT | |
| Cathepsin B | CTSB_F | CTGTCTTGCTGTGGTCTGG |
| CTSB_R | AGGTTGTCACGCATTTGG | |
| Beta-actin | ACTB_F | CTCAACCCCAAAGCCAATC |
| ACTB_R | GAAGCATACAGGGACAGCA | |
| Hemopexin | HPX_F | CGCTCGGATCAGCTCTACAC |
| HPX_R | TTCAGGTCCACCAAAGAAAGG | |
| Integrin beta-4 | ITGB4_F | TTCGCTTGACCAATGATGTAGA |
| ITGB4_R | GGCTTCGTAGTGAAATGCTGAC | |
| Collagen alpha-1(II) chain | COL2A1_F | CCTGGAACACCTGGCACTG |
| COL2A1_R | TCACCTACGTCACCCCTGTCT |