Fig. 5.

The expression of miR-19b-3p promotes proliferation and chemoresistance but has no effect on invasion of SW480 and RKO cells in vitro. a Endogenous miR-19b-3p expression by qRT-PCR in colon cancer cell lines and normal colonic epithelium cells (*, P < 0.05; **, P < 0.01). b Down-regulation of miR-19b-3p suppressed proliferation phenotype in SW480 cells (left) and RKO cells (right). a and d Matrigel-coated transwell assays showed that down-regulation of miR-19b-3p had no effect on invasive abilities of SW480 (c, left) and RKO (c, right) cells. Representative of the images were shown in (d). Statistical analysis was performed with at least three independent experiments. e and f Down-regulation of miR-19b-3p had little effect on viability in the absence of oxaliplatin but promoted cell apoptosis when treated with oxaliplatin in SW480 (f, top) and RKO cells (f, bottom). Representative images of flow cytometry were shown (e) Statistical analysis was performed with at least three independent experiments