Fig. 6.

Down-regulation of miR-19b-3p suppresses colon cancer growth in vivo. a Cells transfected with the miR-19b-3p inhibitor and negative control of SW480 cells were subcutaneously implanted into the flank of nude mice (n = 6). At day 21, the mice were euthanized. b and c The average tumor size (b) and weight (c) of miR-19b-3p inhibitor tumors was reduced significantly as compared to negative control tumors. The tumor sizes were measured at 5-day intervals as soon as the tumors were measurable. The tumor weight were measured at day 21 after the mice being killed. *, P < 0.05 and **, P < 0.01 by Student’s t-test. d Representative Western blot plots of miR-19b-3p inhibitor and SMAD4 expression pattern from tumor-bearing mice