Fig. 8.

miR-19b-3p mediates proliferation and resistance to oxaliplatin-based chemotherapy via SMAD4. a Western blotting assays showed the inverse relationship between miR-19b-3p and SMAD4 in both SW480 and RKO cell lines. β-actin was acted as internal control. b and c CCk8 assays demonstrated that miR-19b-3p inhibitor-transfected SW480 and RKO cells had an impaired proliferation ability which can be reversed by transfection of SMAD4 shRNA. *, P < 0.05, **, P < 0.01. d, e and f miR-19b-3p inhibitor-transfected SW480 and RKO cells were significantly more sensitive to oxaliplatin in comparison with negative control cells,which can be reversed by transfection of SMAD4 shRNA. The ratio of apoptotic cells in every group was shown *, P < 0.05, **, P < 0.01