TABLE 5.
Characteristics of isolates from the 13 patients studied
| Patient | Isolate | No. of mo since first isolatea | DSTb | MIC, μg/ml (day 3/day 14)c | Colony morphotyped | erm(41)e | rrlf |
|---|---|---|---|---|---|---|---|
| 01 | 01-1 | 0 | R | >64 | Mixed (R) | T28 | GA |
| Mixed (S) | T28 | AA | |||||
| 02 | 02-1 | 0 | R | >64 | Mixed (R) | T28 | AA |
| Mixed (S) | T28 | CA | |||||
| 02-2 | 16 | R | >64 | S | T28 | CA | |
| 03 | 03-1 | 0 | R | >64 | S | T28 | AG |
| 04 | 04-1 | 0 | R | >64 | R | T28 | CA |
| 05 | 05-1 | 0 | R | >64 | R | C28 | GA |
| 05-2 | 4 | R | >64 | R | C28 | GA | |
| 05-3 | 6 | R | >64 | R | C28 | GA | |
| 05-4 | 8 | R | >64 | R | C28 | GA | |
| 05-5 | 11 | R | >64 | R | C28 | GA | |
| 05-6 | 19 | R | >64 | R | C28 | GA | |
| 05-7 | 25 | R | >64 | R | C28 | GA | |
| 05-8 | 31 | R | >64 | S | C28 | GA | |
| 06 | 06-1 | 0 | R | >64 | S | C28 | CA |
| 06-2 | 6 | R | 64 | S | C28 | CA | |
| 06-3 | 10 | R | 64 | Mixed (R) | C28 | CA | |
| Mixed (S) | C28 | CA | |||||
| 07 | 07-1 | 0 | R | >64 | R | T28 | TA |
| 07-2 | 3 | R | >64 | R | T28 | GA | |
| 07-3 | 5 | R | 64 | R | T28 | GA | |
| 07-4 | 7 | R | >64 | R | T28 | GA | |
| 07-5 | 14 | R | >64 | R | T28 | GA | |
| 08 | 08-1 | 0 | R | >64 | R | T28 | CA |
| 08-2 | 8 | R | >64 | R | T28 | CA | |
| 08-3 | 9 | R | >64 | R | T28 | CA | |
| 08-4 | 13 | R | >64 | R | T28 | CA | |
| 09 | 09-1 | 0 | R | >64 | Mixed (R) | T28 | CA |
| Mixed (S) | T28 | CA | |||||
| 09-2 | 2 | R | >64 | Mixed (R) | T28 | CA | |
| Mixed (S) | T28 | CA | |||||
| 09-3 | 4 | R | >64 | Mixed (R) | T28 | CA | |
| Mixed (S) | T28 | CA | |||||
| 10 | 10-1 | 0 | R | 64 | R | T28 | AG |
| 10-2 | 2 | R | 64 | Mixed (R) | T28 | AG | |
| Mixed (S) | T28 | AG | |||||
| 11 | 11-1 | 0 | R | >64 | R | T28 | GA |
| 11-2 | 1 | R | 32 | R | T28 | GA | |
| 12 | 12-1 | 0 | R | >64 | R | T28 | AG |
| 12-2 | 1 | R | >64 | S | T28 | CA | |
| 12-3 | 5 | R | >64 | S | T28 | CA | |
| 12-4 | 15 | R | >64 | S | T28 | CA | |
| 12-5 | 18 | R | >64 | S | T28 | CA | |
| 13 | 13-1 | 0 | R | >64 | R | T28 | GA |
| 13-2 | 2 | R | >64 | R | T28 | CA | |
| 13-3 | 7 | R | >64 | R | T28 | CA |
a
The first isolate was a clinical isolate that was firstly confirmed to be resistant to macrolide on DST.
b
For DST: R, resistance.
c
The microdilution method was used to determine the MIC for clarithromycin.
d
For colony morphotype: S, smooth; R, rough.
e
Numbering system of erm(41), with the GTG start codon as 1.
f
Nucleotides 2058 and 2059 (E. coli numbering) of rrl, for which the wild-type sequence is AA. A, adenine; G, guanine; C, cytosine; T, thymine.