FIG 3.

AmB treatment increases transcripts of CAT-encoding genes in ATS and ATR strains. Cultures (for ATS strains, n = 3; for ATR strains, n = 3) were incubated in RPMI 1640 liquid medium for 24 h at 37°C and 200 rpm. AmB (1 μg/ml) was added for 1 h and 4 h. Gene expression during AmB treatment was determined by real-time RT-qPCR, and expression was normalized to that of beta-tubulin according to the 2^−ΔΔCT method. Data are presented as means ± standard deviations for three independent experiments with ATS (white; n = 3) and ATR (black; n = 3) isolates, with technical duplicates. Lines over columns display significant changes between the respective columns (P = 0.05; two-way ANOVA and the Fisher LSD test).