FIG 2.

Bacteria exhibited different infection activities on pHepc-treated IPEC-1 cells. Epithelial cells were preincubated with 16 μg/ml of pHepc or pHepcC-A for 1 h. The bacteria were then allowed to infect the cells (MOI, 10) for 1 h. The relative bacterial adherence or invasion (number of CFU) for E. coli K88 (A) and S. Typhimurium (B) infections is shown. For E. coli K88 infection, cells were washed with PBS to remove nonadherent bacteria, while for S. Typhimurium infection, cells were washed with PBS containing gentamicin. Cell lysates were then used to quantitate the adhering or intracellular bacteria (expressed on the y axis as a ratio of the level for the control group). Cells were then washed and lysed to quantitate adhering and intracellular bacteria (expressed as a percentage of the value for the control group). Error bars indicate SEs. *, P < 0.05.