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pHepc increased the intracellular iron concentration. (Left) 3D4/2 macrophages were infected with S. Typhimurium (STM) for 1 h with or without pretreatment with 16 μg/ml pHepc before the detection of fluorescence. (Right) Phen Green FL was added for 1 h before the amount of intracellular iron was determined by confocal laser scanning microscopy on the basis of the recorded PG-FL fluorescence intensity. Experiments were repeated a minimum of 3 times. Error bars indicate SEs. *, P < 0.05.
