. 2017 Sep 22;61(10):e00272-17. doi: 10.1128/AAC.00272-17

TABLE 2.

Antibiotic resistance profiles and single nucleotide polymorphisms present in MRSA antiseptic-selected mutants compared to their corresponding wild-type strains

Strain	Antiseptic selection^a	MIC (μg/ml)		Antibiotic susceptibility pattern (MIC [μg/ml])^b	ST	Gene name	SNP^c	Amino acid change	Gene function
Strain	Antiseptic selection^a	Polyhex	Chlorhex	Antibiotic susceptibility pattern (MIC [μg/ml])^b	ST	Gene name	SNP^c	Amino acid change	Gene function
COL		0.5	2	Pen^r, Oxa^r, AC^r, Cef^r, Van^s (1.5), Tei (1.5), Dap (0.5)	250
COL P3	Polyhex	2	2	Pen^r, Oxa^r, AC^r, Cef^r, Van^r (3), Tei^r (3), Dap^r (3)	250	mprF	C1010T	L337S	Phosphatidylglycerol lysyltransferase
COL P5	Polyhex	4	2	Pen^r, Oxa^r, AC^r, Cef^r, Tei^r (4), Dap^r (4)	250	mprF	C1010T	L337S	Phosphatidylglycerol lysyltransferase
134947		1	4	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r	228
134947 P6	Polyhex	8	4	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r, Dap^r (1.5–2)	228	purR	T686G	V229G	Pur operon repressor
						mprF	C884T	S295L	Phosphatidylglycerol lysyltransferase
128822		1	8	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r, Gen^r	228
128822 P6	Polyhex	1	8	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r, Gen^r	228	ND^d
COL		0.5	2	Pen^r, Oxa^r, AC^r, Cef^r	250
COL P10	Chlorhex	0.5	8	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r (D17)	250		T to A		Intergenic region (position 775770) between SACOLSACOL_RS03870 and NorA
							G to A		Intergenic region (position 814686) between SACOL_RS04065 and NrdL
							T to A		Intergenic region (position 1227842) upstream SACOL_RS06240
							G to A		Intergenic region (position 1227910) upstream SACOL_RS06245SACOL_RS06245
COL P7	Chlorhex	0.5	8	Pen^r, Oxa^r, AC^r, Cef^r	250	mepA	C127T	T376I	Multidrug export protein MepA
						purR	G403T	V135F	Pur operon repressor
134947		1	4	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r	228
134947 P10	Chlorhex	1	8	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r	228	ND
128822		1	8	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r, Gen^r	228
128822 P10	Chlorhex	1	8	Pen^r, Oxa^r, AC^r, Cef^r, Cip^r, Cli^r, Ery^r, Gen^r, Dap^r (2)	228	pldB	G89T	G30V	Lysophospholipase L2
						glpD	C293A	T98K	Aerobic glycerol-3-phosphate dehydrogenase
						mprF	C1001T	P334L	Phosphatidylglycerol lysyltransferase

Polyhex, polyhexanide; Chlorhex, chlorhexidine.

Antibiotic susceptibility was measured by disc diffusion assays for all antibiotics except vancomycin, teicoplanin, and daptomycin (D corresponds to diameter size measurement; EUCAST ciprofloxacin susceptible diameter = 19–20). For vancomycin, teicoplanin, and daptomycin, MIC values were determined by Etest assays. EUCAST susceptibility breakpoints for are ≤2 μg/ml for vancomycin, ≤2 μg/ml for teicoplanin, and ≤1 μg/ml for daptomycin. Changes in antibiotic resistance patterns compared to those in wild-type strains are indicated by bold type. Pen, penicillin; Oxa, oxacillin; AC, amoxicillin-clavulanate; Cef, cefoxitine; Cip, ciprofloxacin; Cli, clindamycin; Ery, erythromycin; Gen, gentamicin; Van, vancomycin; Tei, teicoplanin; Dap, daptomycin; r, resistant; s, susceptible.

SNP, single-nucleotide polymorphism.

ND, not determined.