Skip to main content
. Author manuscript; available in PMC: 2017 Sep 23.
Published in final edited form as: Mol Cell. 2017 Jun 15;67(2):228–238.e5. doi: 10.1016/j.molcel.2017.05.022

Figure 1. Cell-Free Production of CircRNA.

Figure 1

(A) CircRNA was synthesized by in vitro transcription from a permuted intron-exon template. RNase R treatment of the transcription products enriched for circRNA. Green arrows represent the convergent qRT-PCR primers to detect both linear and circRNA; brown arrows represent the divergent qRT-PCR primers to detect circRNA.

(B) Linear and circRNA were treated with RNase R and analyzed by Bioanalyzer.

(C) Sequencing of circRNA product reveals precise and uniform splice junction.