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. Author manuscript; available in PMC: 2017 Sep 23.
Published in final edited form as: Mol Cell. 2017 Jun 15;67(2):228–238.e5. doi: 10.1016/j.molcel.2017.05.022

Figure 6. Splicing Mechanism Determines Self versus Non-self CircRNA.

Figure 6

(A) Schematic for DNA-programmed splicing of linear or circular ZKSCAN1 or td-directed RNA with endogenous ZKSCAN1 or GFP-IRES exons.

(B) Heatmap of associated proteins identified by ChIRP-MS for each of the spliced RNAs. Enlarged portion of the heatmap shows the proteins for the most enriched circZKSCAN1 RNAs generated by ZKSCAN1 introns. Proteins shown in bold are found enriched in ZKSCAN1 intron-directed circRNAs (both ZKSCAN1 and GFP-IRES exons).

(C) Proteomic analysis of linear and circRNA ribonucleoprotein complexes. Venn diagrams show enriched proteins detected by ChIRP-MS of ZKSCAN1 intron-directed linear or circular ZKSCAN1 or GFP-IRES exons. Gene ontology enrichment for the proteins detected in both linear and circular ZKSCAN1 or GFP-IRES samples.

(D) Model of self versus non-self discrimination of mammalian circRNAs.