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. Author manuscript; available in PMC: 2017 Sep 23.
Published in final edited form as: J Leukoc Biol. 2016 Jul 28;101(2):367–376. doi: 10.1189/jlb.4HI0316-157R

Figure 4. Csf2ram1Kopf develop fatal pulmonary alveolar proteinosis during the course of influenza infection.

Figure 4

Groups of mice were infected with 50 PFU influenza virus PR8 before mice were sacrificed for analysis at the time points indicated. (A) BAL cells were harvested at day 9 p.i. before being analyzed by cytospins (left panel, 10x) and FACS. (B) Panels show H&E-stained histological sections of naïve and day 9-infected mice (10x). (C) BAL fluid was obtained at the time points indicated. Total protein content in BAL fluid was determined by BCA Protein assay (Thermo Scientific) according to the manufacturer’s instructions. Cholesterol levels were measured as described [15] All experiments have been performed at least twice with n = 4 per group.