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. Author manuscript; available in PMC: 2017 Sep 23.
Published in final edited form as: J Am Chem Soc. 2016 Jun 29;138(27):8324–8327. doi: 10.1021/jacs.6b02469

Figure 6.

Figure 6

AFMN synthase catalyzes the formation of formyl-FMN and AFMN in the absence and presence of glutamate. (A) HPLC analysis of the AFMN synthase-catalyzed oxidation of FMN followed by derivatization with pentafluorobenzyl hydroxylamine (PFBHA, blue trace, no glutamate and red trace, no enzyme control). The peaks eluting after 32.2 and 33 min correspond to the two PFBHA oxime isomers. (B) HPLC analysis of the AFMN synthase-catalyzed oxidation of FMN in the presence of glutamate (blue trace, full reaction and red trace, no enzyme conrol).