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Comparison of the UV–vis spectra of formyl and AFMN generated in the AFMN synthase-catalyzed reaction with authentic standards. (A) Overlay of the UV–vis spectra of enzymatically formed formyl-FMN (7, blue trace), formyl-lumiflavin (4, red trace), and FMN (6, green trace). (B) Overlay of the UV–vis spectra of enzymatically formed AFMN (8, green trace), formyl-FMN (7, red trace), amino-lumiflavin (5, blue trace).
