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. Author manuscript; available in PMC: 2017 Sep 23.
Published in final edited form as: J Am Chem Soc. 2016 Jun 29;138(27):8324–8327. doi: 10.1021/jacs.6b02469

Figure 9.

Figure 9

LC-MS analysis of the α-ketoglutarate (24) PFBHA oxime generated from glutamate (17) in the AFMN synthase-catalyzed reaction (negative mode). Left, EIC at m/z 340.0244 for the PFBHA trapped α-ketoglutarate (red trace, trapped α-KG formed in the enzymatic reaction; blue trace, trapped α-KG standard; and green trace, co-elution of trapped α-KG standard and same formed in the AFMN synthase-catalyzed reaction. Right, ESI-MS of the 340.0244 Da species consistent with the α-ketoglutarate PFBHA oxime.