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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Kidney Int. 2017 May 26;92(4):909–921. doi: 10.1016/j.kint.2017.03.027

Figure 1.

Figure 1

Podocyte-specific JAK2 trangene strategy. A loxP-stop-loxP JAK2 construct was inserted into the ROSA26 gene locus using the pBigT/pROSA26PA targeting system and electrodporated into W4 mouse ES cells derived from 129S6 mice. A gene targeted ES cell clone was established and used to produce germline transmitting ES cell-mouse chimeras. Mice carrying the loxP-stop-loxP JAK2 allele were then bred to podocin (NPHS2) Cre mice on a 129S6 background. Podocyte-specific Cre recombinase then excised the floxed stop cassette containing the quadruplicate polyadenylation sequence (4X pA) resulting in transcription of the recombinant JAK2 cDNA driven by the endogenous ROSA26 promoter.