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. 2017 Sep 19;8:1150. doi: 10.3389/fimmu.2017.01150

Figure 2.

Figure 2

In vitro assay to evaluate cytotoxicity induced by the combination of cell lines harboring combination of immunomodulators. Graphs represent GFP-positive cells that were counted using a high-content imaging system. We tested combinations of cell lines that harbored immunomodulators. The immunomodulators are indicated above the graphs. Immunomodulatory B16-GFP cells were cocultivated as indicated below each graph. CM, complete medium; CD4, primed CD4 T cells; CD8, primed CD8 T cells; SP, non-primed splenocytes. RCCs: relative cell counts normalized to the counts in the CM control. Graphs of mean and SEM. ANOVA and Dunnett’s multiple comparisons against CM (*P ≤ 0.005). F10: parental B16F10 cells. RCCs: relative cell counts normalized to the counts in the CM. Results from three independent experiments performed in triplicates.