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Effects of OX-LDL and Sal A on RPE cell junctions. ARPE-19 cells were classified and treated as before. ZO-1 was stained after treatment with SFM, OX-LDL (100 mg/L), and OX-LDL (100 mg/L) + Sal A (5 μM/50 μM) for 48 hours. Representative images of ZO-1 fluorescence showing that OX-LDL induced RPE cell junction disruption and Sal A pretreatment protected cell junction integrity. Scale bar = 50 μm.
