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Brown et al. showed that CYP2B1/2, the rat homolog of human CYP2B6, is maintained for at least 39 days in hepatocyte spheroids cultured under physiologic levels of shear stress in rotating wall vessels. CYP2B1/2 activity was measured by generation of pentoxyresorufin-O dealkylase as pmol per total protein in the culture. Adapted from [5] with permission.
