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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biochem Pharmacol. 2017 Jul 11;143:25–38. doi: 10.1016/j.bcp.2017.07.007

Fig. 4. Effect of DPI analogs on cellular respiration and cell metabolism in HT-29 cells over 24 h.

Fig. 4

(A, B) Concentration-dependent changes in cellular respiration and glycolytic activity following 24-h exposure to DPI (A) or compound 392 (B) were evaluated by measuring oxygen consumption rates (OCR) and extracellular acidification rate (ECAR), respectively, with the Seahorse Extracellular Flux Analyzer. Baseline OCR and ECAR were 383.00 ± 73.65 pMoles/min and 1.19 ± 0.31 mpH/min (A); and 485.00 ± 120.81 pMoles/min and 0.53 ± 0.09 mpH/min (B), respectively. All data represent the mean ± SD of at least three experiments. (C, D) The short-term effects of KCN (2 mM) and a high concentration DPI (2 μM) on OCR (C) and ECAR (D) was also measured in HT-29 cells over 100 min with the Seahorse analyzer. When added to KCN-treated HT-29 cells after 40 min of KCN exposure, DPI (black arrow) recapitulated the effect of KCN.