Table 3.

Effects of DPI analogs on superoxide and H₂O₂ production in human cells. PMA-induced superoxide production was measured by luminescence assay in HEK293 NOX1, PMNs (NOX2), HL-60 (NOX2), and UACC-257 (NOX5) cells. Hydrogen peroxide production was measured by Amplex Red assay of DPI or analog-treated NOX4 and DUOX2/DUOXA2 overexpression cell lines. The cells were incubated with DPI analogs for 30 min prior to assay measurement. The data represent the mean ± standard deviation (SD) of at least 3 independent experiments.

	Superoxide (O2•−)				Hydrogen peroxide (H2O2)
	HEK293 NOX1	PMN (NOX2)	HL-60 (NOX2)	UACC-257 (NOX5)	HEK293 NOX4	HEK293 DUOX2/DUOX A2
Baseline ROS producti on rate	9.88 × 10−2 RLU/min/c ell	0.55 RLU/min/c ell	1.01 RLU/min/c ell	1.34 × 10−2 RLU/min/c ell	2.0 × 10−4 RFU/min/c ell	1.0 × 10−4 RFU/min/cell
DPI IC50 (nM)	233 ± 122	180 ± 36	105 ± 55	42 ± 27	880 ± 214	6,488 ± 1,626
104 IC50 (nM)	70 ± 57	481 ± 44	127 ± 20	44 ± 21	161 ± 153	4,136 ± 1,724
140 IC50 (nM)	249 ± 116	177 ± 60	71 ± 15	55 ± 20	127 ± 110	1,990 ± 1,690
392 IC50 (nM)	357 ± 268	850 ± 197	310 ± 53	208 ± 129	139 ± 143	225 ± 24
428 IC50 (nM)	136 ± 81	324 ± 144	57 ± 35	35 ± 21	260 ± 254	412 ± 236
521 IC50 (nM)	1,019 ± 361	1049 ± 126	1,470 ± 308	229 ± 123	5,533 ± 2,681	773 ± 362

PMN, polymorphonuclear leukocytes.