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. Author manuscript; available in PMC: 2018 Sep 15.
Published in final edited form as: J Mol Biol. 2017 Aug 14;429(19):2954–2973. doi: 10.1016/j.jmb.2017.08.003

Figure 3. Analysis of selected chimeric rabbit/human anti-human ROR1 and ROR2 Fabs by flow cytometry.

Figure 3

(A) The binding of 13 purified chimeric rabbit/human anti-human ROR1 Fabs (10 μg/mL) to ectopically and stably expressed human ROR1 or human ROR2 on mouse pre-B cell line 63-12 and to parental 63-12 cells was analyzed by flow cytometry. Chimeric rabbit/human anti-human ROR2 Fab ERR2-TOP35 was used as control. (B) The binding of 12 purified chimeric rabbit/human anti-human ROR2 Fabs (10 μg/mL) to ectopically and stably expressed human ROR2 allotypes T245 and A245 and mouse ROR2 on HEK293F cells was analyzed by flow cytometry. Mock transfected HEK293F cells were used as control.