Table 1.
Properties of chimeric rabbit/human anti-human ROR1 Fabs.
| HCDR3 | Isotype | LCDR3 | Kd(nM)a | Epitopeb | mROR1c | Westernd | IHCe | |
|---|---|---|---|---|---|---|---|---|
| XBR1-402 | DHPTYGMDL | λ | QVWDSSAYV | 5.81 | Ig+Fz | − | ++ | + |
| ERR1-301 | DHPSYGMDL | λ | QLWDSSAVAYV | 118 | Ig+Fz | − | NA | NA |
| ERR1-306 | WVYGVDDYGDGNWLDL | λ | GTDYSGGYV | 9.66 | Ig+Fz | + | NA | ++ |
| ERR1-316 | DHPNYGMDL | λ | QLWDSSTGAYA | 121 | Ig+Fz | − | NA | NA |
| ERR1-324 | GTVSSDI | κ | LGGYVSQSYRAA | 108 | Ig+Fz | − | + | NA |
| ERR1-403 | GSDYFDL | λ | QLWDSSAGAYV | 41.6 | Ig+Fz | − | NA | NA |
| ERR1-409 | SYPGWTTEPYFDI | λ | LSSDSSAYV | 281 | Ig+Fz | − | NA | NA |
| ERR1-TOP4 | GWATDGIFDYDDTFNL | λ | QLWDGSAYV | 240 | Ig+Fz | − | NA | NA |
| ERR1-TOP15 | DGWGSDV | λ | QLWDSSAGAYV | 139 | Ig+Fz | − | NA | NA |
| ERR1-TOP22 | DLDYDMDL | λ | QLWDNNAAV | 110 | Ig+Fz | + | NA | NA |
| ERR1-TOP40 | LYVSDSTANL | κ | LGGYPNYFHRTA | 636 | Ig+Fz | − | NA | NA |
| ERR1-TOP43 | DVHSTATDL | λ | QLWDSSAGAYV | 1.11 | Ig+Fz | − | +++ | + |
| ERR1-TOP54 | DFGLSTGGL | λ | QLWDSSARAFV | 145 | Ig+Fz | − | NA | NA |
b
Determined by ELISA using hFc fusion proteins of hROR1 whole ECD (Ig+Fz+Kr), tandem domains (Ig+Fz and Fz+Kr), and single domains (Ig, Fz, and Kr).20
c
Cross-reactivity with mouse ROR1; Suppl. Fig. S4.
d
Determined with chimeric rabbit/human IgG1 using purified hROR1-HIS protein and hROR1-expressing K562 cells; purified hROR2-HIS protein and parental K562 cells were used as controls; NA, not analyzed.
e