Table 2.
Properties of chimeric rabbit/human anti-human ROR2 Fabs.
| HCDR3 | Isotype | LCDR3 | Kd (nM)a | Epitopeb | hROR2 SNPc | mROR2d | cROR2e | Westernf | |
|---|---|---|---|---|---|---|---|---|---|
| XBR2-401 | DWTSLNI | κ | LGGYADASYRTA | 3.87 | Kr | + | + | + | +++ |
| XBR2-416 | GIGGAADL | κ | LGGYPNTSYRSA | 348 | Kr | + | − | NA | NA |
| XBR2-433 | DGYYSSGWGPYFNI | λ | ATSGGSGSNPQYV | 2280 | Fz | + | + | NA | NA |
| XBR2-327 | DYGNWAFDP | κ | LGGYASASYRTA | 57.2 | Kr | + | + | + | - |
| XBR2-TOP9 | GGYASGFNL | κ | LGWHSWSDDGWA | 142 | Kr | + | − | NA | NA |
| XBR2-TOP72 | AGTTYYTSFNL | λ | GADYSGGYV | 4150 | Fz+Kr | + | + | NA | NA |
| ERR2-302 | SPYGYVSAWGYHRLDL | λ | QLWDGSDVV | 52.5 | Ig+Fz+Kr | + | + | + | NA |
| ERR2-308 | GASNGCDL | κ | LGGYSNSDIV | 10.6 | Kr | + | − | + | NA |
| ERR2-316 | YFGWNTGFDI | κ | LGFSVVSNDGWA | 5.34 | Kr | + | − | + | NA |
| ERR2-317 | GSSWTGDI | κ | LGGYASASYQTA | 159 | Kr | + | _ | + | NA |
| ERR2-TOP2 | ELVAGGGSDL | κ | LGGYLSASYQTA | 81.1 | Fz+Kr | + | + | NA | NA |
| ERR2-TOP35 | DGYYSSGWGPYFSI | λ | ATSDGSGSRYYV | 43.3 | Fz+Kr | + | + | + | NA |
b
Determined by flow cytometry using cell surface hROR2 whole ECD (Ig+Fz+Kr), tandem domains (Ig+Fz and Fz+Kr), and single domains (Ig, Fz, and Kr) expressed on HEK293 cells.
c
Cross-reactivity with human ROR2 allotypes; Fig. 3B.
d
Cross-reactivity with mouse ROR2; Fig. 3B.
e
Cross-reactivity with cynomolgus ROR2; determined with chimeric rabbit/human IgG1; NA, not analyzed.
f
Determined with chimeric rabbit/human IgG1 using purified hROR2-HIS protein and hROR2-expressing 63-12 cells; purified hROR1-HIS protein, hROR1-expressing 63-12, and parental 63-12 cells were used as controls; NA, not analyzed.