Table 1.
Conservation of the residues selected for mutagenesis across species
| Location | Residue | Rhesus monkey |
Cynomolgus monkey |
Mouse | Hamster | Rat | Chicken | C. elegans |
|---|---|---|---|---|---|---|---|---|
| TMH1 | F72 | ● | ● | ● | ● | ● | ● | |
| TMH5 | F303 | ● | ● | ● | ● | ● | ||
| TMH5 | I306 | ● | ● | ● | ● | ● | ||
| TMH5 | F314 | ● | ● | ● | ● | ● | ● | ● |
| TMH6 | F336 | ● | ● | ● | ● | ● | ● | |
| TMH6 | L339 | ● | ● | ● | ● | ● | ● | |
| TMH7 | F732 | ● | ● | ● | ● | ● | ● | ● |
| TMH8 | F759 | ● | ● | ● | ● | ● | ● | ● |
| TMH8 | F770 | ● | ● | ● | ● | ● | ||
| TMH11 | F938 | ● | ● | ● | ● | ● | ● | |
| TMH11 | F942 | ● | ● | ● | ● | ● | ● | |
| TMH11 | M949 | ● | ● | ● | ● | ● | ||
| TMH12 | L975 | ● | ● | ● | ● | ● | ||
| TMH12 | F983 | ● | ● | ● | ● | ● | ● | |
| TMH12 | F994 | ● | ● | ● | ● | ● | ● |
The residues in human ABCB1 (MDR1) were compared with isoforms in other species, which have higher sequence similarity. The filled circle denotes conservation. TMH, transmembrane helix.