Table 2.

Steady-state transport function of 15Y mutant P-gp with seventeen fluorescent substrates

Fluorescent substrate	% Transport (mean ± SD)	Transport efficiency
TMR Chloride	121 ± 5	Full transport (>75%)
BD-Verapamil	116 ± 4
BD-EDA	108 ± 9
TMRE	100 ± 3
DiOC	94 ± 3
BD-Forskolin	93 ± 12
Calcein-AM	88 ± 15
Cell Tracker Orange	83 ± 6
SYTO 13	82 ± 5
Daunorubicin	79 ± 11
BD-Prazosin	71 ± 7	Partial transport (30–75%)
LDS-751	70 ± 11
Rhodamine 123	66 ± 5
Dihydrorhodamine	63 ± 12
BD-Paclitaxel	29 ± 15	Little to no transport (<30%)
NBD-CsA	22 ± 9
BD-Vinblastine	No detectable transport

Substrates were grouped into three categories based on their transport level when compared to WT; Full transport (>75%), Partial transport (30–75%), and little to no transport (<30%). The transport assays were carried out as described in the Materials and Methods section, and mean ± SD values are from four or more independent experiments. TMR, tetramethylrhosamine, BD-EDA, Bodipy-FL-4,4-difluoro-5,7-dimethyl-4-bora-3a,4a–diaza-s-indacene-3-propionyl ethylenediamine, hydrochloride. TMRE, tetramethylrhodamine ethyl ester perchlorate. DiOC2, 3,3’-Diethyloxancarbocyanine iodide. Cell tracker orange CMTMR, (5-(and-6)-(((4-chloromethy)benzyol)amino)tetramethylrhodamine). LDS-751, quinolinium, 6-(dimethylamino)-2-[4-[4-(dimethylamino)phenyl]-1-ethyl, perchlorate. NBD-CsA, [N-ε(4-nitrobenzofurzan-7-yl)-d-lys cyclosporine A.