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. 2017 Sep 25;8:587. doi: 10.1038/s41467-017-00597-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Ectopic expression of HCN2 rescues the BR⁻ muscle phenotype. a Embryos were microinjected (Inj) with HCN2 mRNA (wild-type channel, WT) either in the two cells (HCN2 WT-group, turquoise arrows) or in one cell (1/2 HCN2 WT, see b blue arrow) at the two-cell stage. Brain was removed at stage 25, and animals with and without brain (Ctrl and BR⁻, respectively) were analyzed for muscle structure and patterning at early- (stages 30–41) and late-stage (stages 42–48). b 1/2 HCN2-WT injection: embryos were microinjected with HCN2 and lacZ mRNA in one of the cells at two-cell stage (blue arrow). The injection side was confirmed by enzymatic detection of β-galactosidase, β-gal (dorsal view of one Ctrl animal is showed on the right). Rostral is up. Scale bar, 1 mm. c Quantification of the mean percentage of abnormal embryos (macroscopic phenotype) and statistical comparisons between uninjected BR⁻ embryos (No Inj) and the different injected-BR⁻ populations (Water, black arrows: water-injection in the two cells; HCN2: HCN2-WT mRNA injection in the two cells; 1/2 HCN2 + β-gal: co-injection of HCN2-WT and lacZ gene reporter in one LR side). Values are plotted as mean % ± s.d. (no-pooled data from two different replicates). d, e Typical muscle phenotype for uninjected BR⁻ (d) and HCN2-WT injected BR⁻ (e), as seen under polarized light. Rostral is upper right and dorsal is up. Scale bar, 100 μm. f, g Quantification of the mean length of myotome fibers and statistical comparisons among uninjected Ctrl and uninjected BR⁻ (BR⁻), HCN2-WT injected BR^- and 1/2 HCN2-WT (measured on uninjected contralateral side) at early- (f one-way ANOVA, P < 0.01) and late- (g one-way ANOVA, P < 0.01) stages after brain removal. No significant differences after a posteriori analysis were detected among the different Ctrl groups. Data represent the mean and s.d. of two independent replicates. For all panels, number in bars indicates n or number of animals for each group. P values after after z-test c or post-hoc Bonferroni’s test f, g are indicated as **P < 0.01, ns P > 0.05