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. Author manuscript; available in PMC: 2017 Dec 26.
Published in final edited form as: Obesity (Silver Spring). 2017 Jun 26;25(8):1410–1420. doi: 10.1002/oby.21900

Figure 6. In male mice, double knockout of Cx3cr1 and Ccr2, but not their individual deletion, reduced M1-like but increased M2-like macrophages in epididymal adipose tissue.

Figure 6

A: Gating strategies for flow cytometric analysis of the stromal vascular fraction of epididymal adipose tissue was performed in male mice. B: CD45+ adipose leukocytes as a percentage of live single cells were not statistically different between groups, but trended lower in Cx3cr1−/−Ccr2−/− mice vs. WT mice. C: F4/80+CD11b+ adipose macrophages as a percentage of live CD45+ cells were not different between groups. D: CD11c+MGL1− M1-like macrophages as a percentage of live CD45+F4/80+CD11b+ macrophages were lower, while CD11c−MGL1+ M2-like macrophages were higher in the Cx3cr1−/−Ccr2−/− mice vs. WT mice. In contrast, a single knockout of Cx3cr1 or Ccr2 did not affect macrophage proportions in adipose. Data are mean±SEM of representative data from 9 WT, 8 Ccr2−/−, 9 Cx3cr1−/−, 8 Cx3cr1−/−Ccr2−/−. *, P<0.05 vs. male WT mice.