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. 2017 Sep 20;11:66. doi: 10.3389/fncir.2017.00066

Figure 6.

Figure 6

Inhibition of central NE release by systemic clonidine and its effects on expression of DRG P2X3 and development of empathic mechanical pain hypersensitivity in the CO rats. (A) showing serum NE levels in CC (n = 7) and CO rats with pre-treatment of i.p. saline (n = 6) or clonidine (n = 7), following 30 min social interaction. (B,C) showing representative immunoblotting bands and quantitative analysis of P2X3 expression in the L4-L5 DRG neurons of the CC and CO rats with pre-treatment of i.p. saline or clonidine (sample n = 3 for each group, however, each sample contains DRGs obtained from at least 9 rats), following social interaction. The relative value density for bands of P2X3 receptors of CO rats was normalized to that in CC rats. (D) showing inhibitory effects of central NE release by systemic clonidine on the development of empathic mechanical pain hypersensitivity (saline, n = 11 and clonidine, n = 7). (E) showing effects of i.p. clonidine (n = 7) on baseline PWMT in the CC rats. CC, cagemate control; CO, cagemate observer; NE, norepinephrine; PWMT, paw withdraw mechanical threshold. †P < 0.05, ††P < 0.01 Saline CO vs. CC; ##P < 0.01 Clonidine vs. Saline for CO; **P < 0.01, ***P < 0.001 vs. pre-treatment. Data are expressed as mean ± SEM.