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. 2017 Sep 20;11:275. doi: 10.3389/fncel.2017.00275

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Copyright © 2017 Murphy, Davila, Cuvelier, Young, Lauderdale, Binder and Fiacco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neuronal swelling persists in Thy1-enhanced green fluorescent protein (eGFP) neurons. (A) Merged infrared (IR) and fluorescent images from a section of the CA1 pyramidal layer in a typical Thy1-eGFP hippocampal slice. GFP-positive neurons are random and generally very sparse, as shown here. (B) Representative thresholded images depicting a Thy1-eGFP neuron at standard imaging depth (~30–40 μm deep) at baseline (B1) and 5 min 40% hACSF time points (B2). Baseline image is overlaid in (B2) to reveal soma size increases (magenta regions, indicated by white arrows). (C) Volume change in neurons exposed to 40% hACSF, labeled either by patch clamp dialysis of dye (“Patch loaded”, Alexa Fluor 488 or 594 hydrazide) or by expression of eGFP.