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. 2017 Sep 20;11:275. doi: 10.3389/fncel.2017.00275

Figure 9.

Figure 9

Astrocyte volume changes do not require expression of aquaporin-4 (AQP4). (A) Representative thresholded images of an AQP4−/− astrocyte soma at baseline (A1), overlaid with an image after 5 min in antagonist-free 40% hACSF (A2) and after 5 min wash in standard ACSF (A3). As in previous figures, magenta regions (indicated by white arrows) represent increases in volume over baseline. (B) Astrocyte volume, quantified as percent change from baseline, in AQP4−/− and wild-type astrocytes exposed to 17% or 40% hACSF. AQP4−/− astrocytes showed no change in swelling at 17% hACSF and significantly greater swelling at 40% hACSF, as compared to wild-type. (C) Representative thresholded images and overlays of an AQP4−/− neuron soma at baseline (C1), after 5 min in antagonist-free 40% hACSF (C2), and after a 5 min wash period in standard ACSF (C3). (D) Volume changes in hACSF are compared between AQP4−/− and wild-type neurons. As expected, neuronal swelling did not differ significantly between these two genotypes. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. wild-type astrocytes in 40% hACSF. Scale bars, 5 μm each for (A,C).