Table 3.

Collection of C-terminal protein fusions for marker-less integration.

Plasmid (pGF)	Marker(s)	Description	Size (amino acids)	References
1204	1xFLAG-Linker (25)-GFP(β11)	Biochemical Tag + Split GFP Taga	54	Hopp et al., 1988; Cabantous et al., 2013
1205	1xFLAG-Linker (25)-SpeI-6xHIS	Biochemical Tagsb	41	Hochuli et al., 1988
1206	mCherry(opt)	Yeast Codon Optimized Fluorescent Proteinc	235	Shaner et al., 2004
1207C	anti-GFP Nanobody	Anti-GFP Protein Tetherd	117	Rothbauer et al., 2006; Kubala et al., 2010
1208	GST	Biochemical Tage	225	Benard and Bokoch, 2002
1209	1xFLAG-Linker(25)-3xHA	Biochemical Tagf	65	Wilson et al., 1984; Field et al., 1988
1302	NLS	Cellular Localizationg	11	Kalderon et al., 1984
1303	NES	Cellular Localizationh	11	Xu et al., 2012
1304	1xMYC	Biochemical Tagi	10	Hilpert et al., 2001; Krauss et al., 2008
1305	MBP	Biochemical Tagj	375	Duplay et al., 1984
1306	BirA(R118G)	Proximity-Dependent Protein Biotinylationk	321	Choi-Rhee et al., 2004; Cronan, 2005
1307	CAAX Box Motif	Cellular Localizationl	9	Mitchell et al., 1994
1309	SNAP tag	Fluorescent Proteinm	182	Juillerat et al., 2003
1310	S. pombe His5	Cellular Growth Screeningn	216	Longtine et al., 1998
1311	mScarlet(opt) WT	Yeast Codon Optimized Fluorescent Proteino	231	Bindels et al., 2017
1379	eGFP(opt)	Yeast Codon Optimized Fluorescent Proteinp	237	Cinelli et al., 2000
1380	ymUkG1(opt)	Yeast Codon Optimized Fluorescent Proteinq	227	Kaishima et al., 2016
1381	eGFP(opt)-LactC2	Yeast Codon Optimized Fluorescent Protein + Membrane tetheringr	395	Andersen et al., 2000; Shao et al., 2008

^aFLAG Epitope, Flexible linker (GSGAGGSPGGGSGGSGSSASGGSTS), GFP(β-11) strand (EKRDHMVLLEYVTAAGITDAS).

^bFLAG Epitope, Flexible linker (GSGAGGSPGGGSGGSGSSASGGSTS), SpeI restriction site (residues TS), and 6x Histidine tag.

^cmCherry(opt); yeast optimization begins at residue 21; CAI (codon adaptation index) value = 0.92.

^dAnti-GFP Nanobody; yeast optimized (CAI value = 0.84)

^eGlutathione S-transferase (GST); sequence begins with “SPILGYW…” and ends with “…DLVPRGS.”

^fFLAG Epitope, Flexible linker (GSGAGGSPGGGSGGSGSSASGGSTS), and 3xHA (Human influenza hemagglutinin) tag (YPYDVPDYAGYPYDVPDYAGSYPYDVPDYACG).

^gNuclear Localization Signal (SRADPKKKRKV) SV40 Large T-antigen.

^hNuclear Export Signal (LAKILGALDIN).

ⁱMYC Epitope (EQKLISEEDL).

^jMaltose Binding Protein (MBP); sequence begins with “KIEEGKL…” and ends with “…NSSSARL”. There is a XhoI (residues LE) restriction site preceding the stop codon.

^kBirA(R118G); 35 kD DNA-binding biotin protein ligase in Escherichia coli.

^lCAAX Box Motif (GSGGCCIIS) from yeast Ras2.

^mSNAP Tag; 20 kDa mutant of the DNA repair protein O6-alkylguanine-DNA alkyltransferase.

ⁿS. pombe HIS5 gene product; Imidazoleglycerol phosphate dehydratase HisB.

^omScarlet-WT(opt) (CAI value = 0.93).

^peGFP(opt); yeast codon optimization begins after residue 9 (CAI = 0.92). Contains S65T, F64L, R88Q, and H239L.

^qymUkG1(opt) yeast codon bias (CAI = 0.92).

^reGFP(opt)-Lact-C2; Bovine Lactadherin C2 Domain (158 residues) binds phosphatidylserine on the inner leaflet of the plasma membrane.