Table 1.
Primers used for the RT-qPCR assays.
| Primer | Sequence (5′→3′) | Target gene | Amplicon length (bp) | Slope | y-intercept | Amplification efficiency | R2 | Reference |
|---|---|---|---|---|---|---|---|---|
| SlonirK755f | TGAGTGAGGTGCTTGAGGTG | nirK | 237 | –3.474 | 35.552 | 94.0 | 0.998 | This study |
| SlonirK991r | TCCAGGTTTCCAGATTGGTC | |||||||
| SlonrfA724f | CGTCATCCTGAGTTTGAGCA | nrfA | 227 | –3.477 | 36.396 | 99.8 | 0.998 | Yoon et al., 2013 |
| SlonrfA950r | TTCTCGGCTATCTGCGACTT | |||||||
| SlorecA656f | ACGCTTCTGTTCGTCTGGAT | recA | 245 | –3.366 | 35.931 | 98.2 | 0.999 | This study |
| SlorecA900r | GCCAATCTTGTCACCCTT | |||||||
| SlonosZ599f | ATGGTAAGGAGACGCTGGAA | nosZ | 160 | –3.386 | 34.713 | 97.4 | 0.999 | This study |
| SlonosZ758r | TTGTAGCAGGTAGAGGCGAAG |