. 2017 Sep 20;8:1818. doi: 10.3389/fmicb.2017.01818

Table 3.

Effect of high grain feeding and thiamine supplementation on relative abundances of bacterial genera in rumen fluid using 16S rRNA sequencing¹ (%).

Phylum	Family	Genus/other	Experimental treatments²			SEM³	P-value
			CON	HG	HG+T
Bacteroidetes	Bacteroidaceae	Bacteroides	0.44^a	0.19^b	0.30^a	0.043	0.034
	Prevotellaceae	Uncultured Prevotellaceae	0.10^a	0.06^b	0.10^a	0.018	0.022
	Unclassified	Unclassified Bacteroidetes	0.53^a	0.45^b	0.64^a	0.027	0.008
Firmicutes	Christensenellaceae	Christensenellaceae R7	1.35^a	2.02^a	0.95^b	0.21	0.011
	Erysipelotrichaceae	Erysipelotrichaceae UCG-004	0.21^a	0.15^b	0.13^b	0.015	0.016
	Fibrobacteraceae	Fibrobacter	1.05^a	0.60^b	0.87^b	0.081	0.046
	Lachnospiraceae	Uncultured Lachnospiraceae	0.79^a	0.38^b	0.32^b	0.14	0.017
		Eubacterium ventriosum	0.13^a	0.06^b	0.08^b	0.016	0.003
		Oribacterium	0.14^b	0.15^b	0.29^a	0.023	0.011
		Lachnospira	0.06^b	0.13^a	0.16^a	0.015	0.014
	Ruminococcaceae	Ruminococcaceae NK4A214	1.71^b	2.73^a	1.52^b	0.16	0.003
		Ruminococcus 1	0.93^a	0.54^b	0.76^a	0.05	0.005
		Ruminiclostridium 5	0.46^a	0.21^b	0.14^b	0.048	0.004
		Saccharofermentans	0.76^a	0.65^a	0.51^b	0.06	0.035
		Papillibacter	0.54^a	0.46^a	0.31^b	0.038	0.018
		Anaerotruncus	0.12^a	0.10^a ^b	0.07^b	0.015	0.050
	Veillonellaceae	Succiniclasticum	8.89^b	12.45^a	9.61^b	1.18	0.035
		Schwartzia	0.14^b	0.18^b	0.35^a	0.026	0.004
		Selenomonas 1	0.65^a	0.30^b	0.49^a ^b	0.075	0.077
Proteobacteria	Acetobacteraceae	Acetobacter	0.17^a	0.01^b	0.008^b	0.023	0.001
	Succinivibrionaceae	Succinivibrionaceae UCG-002	1.62^b	0.30^c	3.13^a	0.15	<0.001
		Unclassified Succinivibrionaceae	0.24^a	0.08^b	0.21^a	0.069	0.016
		Succinivibrio	0.07^a	0.02^b	0.09^a	0.009	0.003
		Ruminobacter	0.09^a	0.02^b	0.06^a	0.01	0.002
Spirochaetae	Spirochaetaceae	Treponema 2	2.77^b	2.74^b	4.22^a	0.14	0.002
Synergistetes	Synergistaceae	Pyramidobacter	0.18^a	0.04^c	0.11^b	0.013	<0.001

^a,b,c

Means values within a row with different letters differ significantly (P < 0.05).

Only bacterial genera (accounted for ≥0.1% in at least one of the samples) that affected by treatments were listed.

CON, control diet; HG, high grain diet; HG+T, high grain diet supplemented with 180 mg thiamine/kg DMI.

SEM, standard error of the mean.