FIGURE 3.

Pre-treatment with LBS decreases E. coli K1-induced damage and apoptosis of HT-29 cells. HT-29 cells were treated as described in Figure 1. (A) LDH activity was analyzed by microtiter plate reader and the results were expressed as a percentage of the control (untreated cells). Results are presented as mean ± SD. ^∗P < 0.05. (B) HT-29 cells were illustrated by fluorescence microcopy (100× magnification). Hoechst 33342 was used to stain nuclei of all cells (blue), PI was used to detect the dead cells (red).