Table 1.
Engineering of plant miRNA for antiviral immunity.
| Plant species | MiRNA backbone | Virus | Target viral region/gene | Reference |
|---|---|---|---|---|
| Arabidopsis thaliana | Arabidopsis pre-miR159 | TYMV, | P69, | Niu et al., 2006 |
| TuMV | HC-Pro (coat protein) | |||
| Nicotiana benthamiana | Arabidopsis miR159a, miR167b, and miR171a | PPV | P1/HC-Pro | Simon-Mateo and Garcia, 2006 |
| Nicotiana benthamiana | Arabidopsis pre-miR171a | CMV | 2b viral gene | Qu et al., 2007 |
| Arabidopsis thaliana | Arabidopsis pre-miR159 | CMV | 3′-UTR | Duan et al., 2008 |
| Arabidopsis thaliana, Nicotiana benthamiana | Arabidopsis pre-miR159 | TuMV | P69 | Lin et al., 2009 |
| Nicotiana tabacum | Arabidopsis miR159a, | PVY | HC-Pro, | Ai et al., 2011 |
| miR167b, and miR171a | PVX | TGBp1/p25 (p25) | ||
| Solanum lycopersicum | Arabidopsis pre-miR159a | CMV | 2a and 2b viral genes, 3′-UTR | Zhang et al., 2011 |
| Nicotiana benthamiana | Arabidopsis pre-miR159a | WSMoV | Conserved motifs of L (replicase) gene (A, B1, B2, C, D, E, AB1E, B2DC) | Kung et al., 2012 |
| Triticum | Rice miR395 | WSMV | Conserved region | Fahim et al., 2012 |
| Vitis vinifera | Arabidopsis pre-miR319a | GFLV | Coat protein (CP) | Jelly et al., 2012 |
| Nicotiana benthamiana | Cotton pre-miR169a | CLCuBuV | V2 gene | Ali et al., 2013 |
| Solanum lycopersicum | Arabidopsis pre-miR319a, Tomato pre-miR319a and pre-miR168a | ToLCV | The middle region of the AV1 (coat protein), the overlapping region of the AV1 and AV2 (pre-coat protein) | Vu et al., 2013 |
| Nicotiana benthamiana Nicotiana. tabacum | Arabidopsis pre-miR319a | PVY | CI, NIa, NIb, CP | Song et al., 2014 |
| Zea mays | Maize pre-miR159a | RBSDV | Conserved region | Xuan et al., 2015 |
| Nicotiana benthamiana | Barley pre-miR171 | WDV | Conserved region | Kis et al., 2016 |
| Oryza sativa | Rice pre-miR528 | RSV, RBSDV | Middle segment, 3′ end and 3′-UTR region of the CP gene | Sun et al., 2016 |
| Nicotiana benthamiana | Arabidopsis pre-miR159a | CBSV, UCBSV | P1, P3, CI, Nib and CP | Wagaba et al., 2016 |
| Nicotiana benthamiana | Arabidopsis pre-miR159a | TSWV | N, NSs | Mitter et al., 2016 |
| Nicotiana benthamiana | Six amiRNAs | PSTVd | Structural domains | Carbonell and Daros, 2017 |
TYMV, Turnip yellow mosaic virus (Potyviridae); TuMV, Turnip mosaic virus (Potyviridae); CMV, Cucumber mosaic virus (Bromoviridae); PPV, Plum pox virus (Potyviridae); PVY, Potato virus Y (Potyviridae); PVX, Potato virus X (Alphaflexiviridae); WSMoV, Watermelon silver mottle virus (Bunyaviridae); WSMV, Wheat streak mosaic virus (Potyviridae); GFLV, Grapevine fan leaf virus (Secoviridae); CLCuBuV, Cotton leaf curl Burewala virus (Geminiviridae); WDV, Wheat dwarf virus (Geminiviridae); RSV, Rice stripe virus (unassigned); RBSDV, Rice black streaked dwarf virus (Reoviridae); CBSV, Cassava brown streak virus (Potyviridae); UCBSV, Ugandan cassava brown streak virus (Potyviridae); TSWV, Tomato spotted wilt virus (Bunyaviridae); PSTVd, Potato spindle tuber viroid (Pospiviroidae); ToLCV, Tomato leaf curl virus (Geminiviridae).