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. 2017 Sep 20;8:1807. doi: 10.3389/fmicb.2017.01807

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Copyright © 2017 Zhu, Wei, Wu, Zhou, Peng, Zhang, Chen and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sequence similarities between BcCFEM1 and other CFEM proteins. (A) Schematic illustrations of BcCFEM1. The domain of BcCFEM1 was predicted by SMART web site. (B) Phylogenetic analysis of BcCFEM1 and CFEM proteins from other fungi. The full length protein sequences were analyzed by MEGA 5 with Unrooted neighbor-joining bootstrap (1,000 replicates). The black triangle indicates BcCFEM1. A scale bar at the lower left corresponds to a genetic distance of 0.1. (C) Multiple sequence alignment of BcCFEM1 and other CFEM proteins. Full-length of protein sequences were aligned using Clustal W and the alignment was edited using Jalview. Eight conserved cysteine residues are highlighted with red triangles. Blue shading intensity reflects level of amino acid identity at each position. Botrytis: BcCFEM1 (BC1G_15201); Sclerotinia: SS1G_07295; Metarhizium: MAM_04868; Phialocephala: PAC_04512; Colletotrichum: CGLO_03584; Trichoderma: TGAM01_02421.