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Dual LUC assays reporting miR529b-mediated repression of a PM target within the firefly reporter gene construct, after a range of incubation periods post co-agroinfiltration. The controls consist of assays where the miR529b precursor construct culture was replaced with a construct that is predicted to form a secondary structure but does not target the mir529b PM sequence. The results are depicted as box and whisker plots, with interquartile range boxes and min/max whiskers (n = 12).
