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Comparison of 4 h versus 24 h acetosyringone incubation periods. Increasing the incubation period of Agrobacterium cultures from 4 to 24 h post-addition of acetosyringone did not enhance the mi529b-directed knockdown of reporter-target expression. The controls consist of assays where the miR529b precursor culture was replaced with a precursor construct culture that does not target the miR529b PM sequence. Error bars represent SDs (n = 12).
