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. 2017 Sep 20;8:1631. doi: 10.3389/fpls.2017.01631

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Copyright © 2017 Moyle, Carvalhais, Pretorius, Nowak, Subramaniam, Dalton-Morgan and Schenk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental validation of rice transcript sequences computationally predicted to be targeted by rice miR529b. Each control consists of assays where the miR529b precursor culture was replaced with a precursor construct culture that does not target the rice sequences. Mismatch1 (MM1) and mismatch2 (MM2) negative control assays use target sequences with little complementarity to the miR529b miRNA. The results are depicted as box and whisker plots, with interquartile range boxes and min/max whiskers (n = 6). Asterisk represents statistically significant differences using a simple two-tailed unpaired t-test with ^∗P < 0.05, ^∗∗P < 0.005, and ^∗∗∗∗P < 0.0001.