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. 2017 Sep 20;8:1630. doi: 10.3389/fpls.2017.01630

FIGURE 6.

FIGURE 6

In situ hybridization analyses of MtARF3 and MtAGO7 gene expression in M. truncatula. (A) Localization of MtAGO7 transcripts in vegetative shoot buds in WT. (B,C) Localization of MtARF3 in vegetative shoot bud (B) and petiole of a developing leaf (C) in WT. Note that in wild type plants, MtAGO7 and MtARF3 expressed in opposite domains in leaf primordia, with MtAGO7 being expressed in the leaf adaxial domain and MtARF3 in the leaf abaxial domain. (D,E) Localization of MtARF3 gene expression in mtago7 mutant. MtARF3 gene expression was detected in both abaxial and adaxial domains of leaf primordia in the mtago7 mutant. (F,G) Localization of MtARF3 gene expression in mtphan mutant. MtARF3 gene expression was detected in both abaxial and adaxial domain of leaf primordia in the mtphan mutant. (H,I) Localization of MtARF3 gene expression in mtphan;mtago7 double mutant. MtARF3 gene expression was detected in both abaxial and adaxial domains of leaf primordia in the mtphan;mtago7 double mutant, with stronger signals in the adaxial domain. (J,K) Negative controls using sense probes in neighboring sections for detection of MtAGO7 (J) and MtARF3 (K). Scale bars: 100 μm. Dashed lines mark the boundary between the adaxial and abaxial domains. SAM, shoot apical meristem; P, plastochron; Ab, abaxial domain; Ab, abaxial domain.