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. Author manuscript; available in PMC: 2018 Jun 6.
Published in final edited form as: Anal Chem. 2017 May 10;89(11):6248–6256. doi: 10.1021/acs.analchem.7b01247

Figure 1.

Figure 1

Schematic comparison of four different sandwich ELISA formats for human sEH detection using the same rabbit polyclonal anti-sEH as capture antibody, (a) format A: native nanobody and HRP labeled anti-HA tag IgG (VHH/HRP-anti-HA), (b) format B: biotinylated nanobody and HRP labeled anti-HA tag IgG (Biotin-VHH/HRP-anti-HA), (c) format C: biotinylated nanobody and streptavidin-polyHRP conjugate (Biotin-VHH/SA-PolyHRP), (d) format D: biotinylated nanobody and streptavidin-HRP conjugate (Biotin-VHH/SA-HRP), as detection antibody and tracer, respectively.