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. Author manuscript; available in PMC: 2018 Jun 6.
Published in final edited form as: Anal Chem. 2017 May 10;89(11):6248–6256. doi: 10.1021/acs.analchem.7b01247

Figure 2.

Figure 2

Signal response of nanobody based ELISA formats toward human sEH using rabbit anti-sEH polyclonal antibody (4.5 μg/mL) as capture antibody: (a) format A: conventional ELISA format using ten native VHH clones (1 μg/mL) as detection antibody and HRP labeled anti-HA tag IgG (200 ng/mL) as the tracer. This graph is plotted based on the data from work of ref 29; (b) format C: PolyHRP based format using biotinylated nanobody (A1 or A9, 1 μg/mL) as the detection antibody and SA-PolyHRP (25 or 50 ng/mL) as the tracer; (c) expanded low concentration range of Figure 2b. Error bars indicate standard deviations (n = 3).