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Comparison of the four ELISA formats with the decreasing working concentrations of (Figure 4a,b) capture serum (1:2,000–1:64,000 dilution) or (Figure 4c,d) detection nanobody (1.0–1/32 μg/mL). The O.D. decreased slightly below 4.0 in higher concentrations than the sEH saturated concentration due to the partial degradation of the over-saturated product of color development (see Figure 4a,c).
